http://www.thehindu.com/sci-tech/article3551216.ece?homepage=true
Molecular and isotope analysis of absorbed food residues on potsherds revealed the details
Researchers from the University of Bristol and other institutions have 
found the “first unequivocal” chemical evidence of dairying practices by
 Saharan people about 5,000 years ago — at a time when the region was in
 a humid phase and had plenty of plant cover. 
The story so far
Researching the earliest evidence of dairying has so far been confined 
to Europe, Near East and Eurasia. This is the first time an attempt is 
made to study African samples. The results are published today (June 21)
 in Nature. 
Till date evidence of domestication of cattle, sheep and goats came from
 faunal samples. But faunal remains have been “highly fragmentary and 
poorly preserved.” Reconstructing evidence of herding has therefore been
 difficult. 
Even indirect evidence of dairying is “missing.” Of course, rock 
paintings and engravings have provided some compelling indirect 
evidence. 
The researchers therefore turned to molecular and isotope analysis of 
absorbed food residues found on potsherds to know the details. The 
rationale is simple: analysing food residues is a sure way of 
understanding diet and subsistence practices of humans a few thousand 
years ago.
Making the study possible has been the exemplary preservation of 
absorbed organic residues, particularly lipids, on potsherds. This is 
unlike in the case of European sites where only 40 per cent of potsherds
 provided any evidence of lipids, and that to at very low 
concentrations. “This remarkable preservation [in the case of African 
samples] is likely to be related to the extremely arid conditions 
prevailing in the region” in the last hundreds of years. 
The researchers used carbon 13 isotopic ratios to study the major 
alkanoic acids of milk fat. The lipids belonged to three categories — 
“high abundance” of C16:0 and C18:0 (lipid numbers) fatty acids derived 
from degraded animals fats. There were carbon isotopes (C13 to C18) 
which are demonstrative of “bacterial origin” and diagnostic of 
“ruminant animal fats.” 
In the second category, the carbon isotopes found were diagnostic of 
plant oils and a certain kind of wax of vascular plants. The third type 
of residue indicates the “drying reaction of plant oils,” and reflects 
either “processing of both plant and animal products in the same vessel 
or the multiuse of the vessels.” 
Of the three types, only those indicative of degraded animal fats were 
taken up for detailed analysis. Compared with present day animal fats, 
about 50 per cent of lipid samples recovered from the potsherds fall 
within or on the edge of isotopic range of dairy fats. About 33 per cent
 fall within the isotope range for ruminant adipose fats.
“The unambiguous conclusion is that the appearance of dairy fats 
correlates with the abundant presence of cattle bones in the cave 
deposits, suggesting a full pastoral economy,” they write. 
They also found unequivocal evidence for “extensive processing of 
dairying products” in pottery in the Libyan Sahara between 5,200-3,800 
years ago. This confirms that “milk played an important part in the diet
 of these prehistoric pastoral people.” 
This is quite surprising considering the fact that these people were 
able to consume milk despite suffering from lactose intolerance. The 
study thus provides a window to the “evolutionary context for the 
emergence of lactase persistence in Africa.”
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